长穗偃麦草转录组分析
Investigation of Transcriptome sequencing in Thinopyrum elongatum
长穗偃麦草是小麦重要的近源物种,含有丰富的抗逆基因,广泛地应用于小麦的遗传改良育种。本研究利用高通量测序,获得长穗偃麦草的转录组信息,利用比较基因组学方法研究其与小麦、水稻和玉米等作物的遗传关系,评估它们之间的亲缘关系。同时,将长穗偃麦草的高通量序列比对到小麦基因,利用软件Freebayes和SAMtools/Bcftools发掘功能基因的变异位点,并对这些含有变异位点的功能基因进行注释分析,揭示长穗偃麦草优异性状形成的分子机制,这将为长穗偃麦草优异基因资源的开发和应用奠定重要基础。
hinopyrum elongatum, an important relative of common wheat, is favored by many researchers because it harbors numerous biotic and abiotic stress-resistance genes. In this study, we used high-through sequencing technology and comparative genome strategies to analyze the Th. elongatum transcriptome, and evaluate evolution relationship between Th. elongatum and other cereal crops. Meanwhile, all sequences were aligned to wheat genes, and SNP sites were identified by software Freebayes and SAMtools/Bcftools. These genes containing SNP sites were annotated using COG, which was helpful for exploring molecular mechanism of excellent traits formation in Th. elongatum, and it provides a valuable reference for future development and utilization of excellent genes in Th. elongatum.
束永俊、刘赢、宋丽莉、张军、敖游
农业科学研究植物学遗传学
作物遗传育种长穗偃麦草转录组分析功能基因单核苷酸多态性
crop genetic breedingThinopyrum elongatumTranscriptomic analysisfunctional genesingle nucleotide polymorphism
束永俊,刘赢,宋丽莉,张军,敖游.长穗偃麦草转录组分析[EB/OL].(2015-04-07)[2025-08-04].http://www.paper.edu.cn/releasepaper/content/201504-103.点此复制
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