高强度聚焦超声对前列腺癌小鼠的疗效及T细胞亚群的影响

Objective To explore therapeutic effects of high intensity focused ultrasound on mice with prostate cancer and its influence on systemic T cell subsets. Methods To establish subcutaneous murine prostate carcinoma model by vaccinating RM-1 cells to C57BL6 mice, mice were randomly divided into control group, radiotherapy group, HIFU group, HIFU combined with radiotherapy group. We observed the growing curve of tumor after treatment and calculated the tumor doubling time (DT). Two weeks later, normal and tumor-bearing mice were observed CD3+, CD3+CD4+ and CD3+CD8+ cells' percentage within the total white blood cell population by flow cytometric analysis. Results Comparing with control group, both HIFU and radiotherapy slow tumor's growth and significantly prolonged the DT. CD3+(%) and CD4+(%) of mice in HIFU group were higher than those in control group, and there was no significant difference between radiotherapy group and control group. The DT of HIFU combined with radiotherapy group was significantly prolonged than that of HIFU group, and its CD3+(%) and CD4+(%) were significantly higher than those of HIFU group, CD4+(%) were significantly higher than those of normal mice. Conclusion HIFU in treatment of prostate cancer not only inhibited local tumor growth, but also improved systemic cellular immunity by influencing T cell subsets. HIFU in combined with radiotherapy further improved its therapeutic effects.