材料表面成分对干细胞成骨分化的影响的研究

he slight difference in biomaterial surfaces has important effect on cells functions. Micropatterning technology make it possible to prepare a surface with different surface properties in regular areas. In this study, micropatterning technology was applied to prepare micropatterns with three kinds of different surface properties on cells culture plate surfaces, and the effect of materials surface physicochemical properties on the osteogenic differentiation of hMSCs was investigated in the same environment. In this study, the photo-reactive polymers were synthesized by the introduction of azidophenyl groups into the side chains of polymers, and the micropatterns were prepared by UV-irradiation of photo-reactive polymers on cells culture plate surface, the height and integrity of immobilized photo-reactive polymers were detected by the optical microscope and scanning probe microscope. hMSCs were cultured on the micropattern surfaces, and performed osteogenic differentiation, ALP staining and Alizarin Red S staining were conducted to detect the effect of surface component on the osteogenic differentiation of hMSCs. The results indicated that micropattern with different surface properties in regular morphology can be prepared by UV-irradiation method, and surface component of micropatterns had important effect on the osteogenic differentiation of hMSCs, gelatin micropatterned areas were beneficial for the deposition of minerals during osteogenic differentiation.