奉晚与奉节72-1脐橙的蔗糖代谢相关酶基因表达

Expression analysis of genes of sucrose-metabolizing enzymes using RT-PCR and change of sugar contents in fruit juice sacs (edible parts) between Fengwan and Fengjie72-1 navel orange (Citrus sinensis L.Osbeck) during fruit maturation were studied in this paper. Results showed that the acid invertase (AI) gene expression in Fengwan orange was different from Fengjie72-1 orange, while sucrose synthase(SS) gene and sucrose phosphate synthase(SPS) gene had no distinct expressing difference between the two cultivars, SS could be detected and SPS could not, during fruit maturation. As the same period, sugar accumulations including sucrose, glucose and fructose in the two cultivars were exhibited difference too. Sugar accumulation in Fengjie72-1 orange presented increasing and decreasing curve, while in Fengwan orange was kept an increasing trend from Oct. to Jan. Before January, Sugar contents in Fengwan orange were obviously lower than those in Fengjie 72-1 orange. Taken together, AI expressing difference might be one of the main reasons of sugar difference between Fengwan orange and its parental line, Fengjie 72-1 orange.