番茄NAM基因的克隆与遗传转化

For understanding the function of NAM gene in tomato, the full length cDNA NAM gene was amplified by RT-PCR from tomato cDNA. It contains a 1,053 bp open reading frame (ORF) encoding 351 amino acid residues, and contains NAC domain in its N terminus. Quantitative PCR analysis showed that NAM expressed highly in all tissues including root, stem, leaf, flower and fruit. To further investigate the function of tomato NAM gene, NAM overexpression vector pLP-35S-NAM was constructed, and transformed into tomato plants by Agrobacterium-mediated method. The NAM overexpressing plants showed a dwarf phenotype, the size of these plants was smaller than those of wild-type plants, the plant growth was inhibited, and the leaves of transgenic tomato was highly lobed shaped. In conclusion, NAM affected the formation of shoot apical meristem (SAM) and leaf morphogenesis in tomato.