基因差异表达参与丹参内在品质杂种优势的分子机制研究

Heterosis describe the phenomenon the superior performance of hybrids relative to parents. It is widely used for production improvement，but the molecular basis is still not fully understood. We observed heterosis for hydrophilic and lipophilic compounds in S. Miltiorrhiza. We used three five generations inbred lines with various hydrophilic content of S. Miltiorrhiza to construct three hydrophilic F1 groups, and three inbred lines with various lipophilic content to generate three lipophilic F1 groups. We investigated transcriptome profiles of 3 hydrophilic groups and 3 lipophilic groups. We detected 2302 and 19Transcriptomic analysis of Salvia miltiorrhiza Bunge hybrids and their inbred parents involved in the heterosis of secondary metabolites31 differentially expressed genes (DEGs) in hydrophilic groups and lipophilic F1 groups, respectively. For both of these compounds, the number of DEGs were in accordance with the degree of heterosis. Clustering results showed that the expression profiles didn't consistent trends with phenotypic observations. Pathway assignments involving biosynthesis of secondary metabolites amongst the 574 and 742 DEGs. The majority of all detected genes were non-differently-expressed, in water-group the number of expression-level dominance and transgressive expression were 1581 and 709, respectively. While in fat-group he number of expression-level dominance and transgressive expression were 780 and 1145. We conclude that non-additive genes are important in heteroses in S. Miltiorrhiza. and contribute specifically to secondary metabolites heterosis.