脱细胞基质增强间充质干细胞的抗氧化功能

Objective: To evaluate the influence of decellularized extracellular matrix (DECM) on antioxidant properties of mesenchymal stem cells (MSCs). Methods: MSCs were stimulated to generated ECM and, after decelluarization, the matrix proteins in the DECM were determined by immunofluoresence staining. MSCs were cultured on DECM and the intracellular reactive oxygen species (ROS) and the gene expression of several antioxidant enzymes were evaluated. Results: the architecture of cell-deposited ECM was characterized as nanofibrous collagen fibrils and the matrix components were identified as type I and III collagens, fibronectin, and laminin. The levels of intracellular ROS in DECM-cultured cells were reduced by 41.7%. Culturing on DECM enhanced the expression of intracellular antioxidative enzymes such as superoxide dismutase and catalase. Conclusions: Collectively, by providing sufficient cell amounts and enhancing antioxidant capacity, DECM can be used as a promising cell culture platform for in vitro expansion of UC-MSCs.