伽马射线对重组质粒(CArG)6-hTERTp-SEA-GPI在人肿瘤A549细胞和成纤维细胞WI-38中的SEA表达的影响
he effect of γ-Ray on the SEA expression in A549 and WI-38 cells transfected with plasmid of (CArG)6-hTERTp-SEA-GPI
目的：观察γ-射线对重组质粒(CArG)6-hTERTp-SEA-GPI在人肿瘤A549细胞和人正常细胞WI-38中的SEA表达的影响。方法：重组质粒(CArG)6-hTERTp-SEA-GPI分别转染A549和WI-38细胞，转染后12小时2GY γ-射线照射肿瘤细胞和正常细胞，转染后24h后采用RT-PCR法检测SEA mRNA的表达，转染后48h采用Western blot 法检测SEA 蛋白的表达。结果：(CArG)6-hTERTp-SEA-GPI能够在肿瘤细胞A549细胞中表达SEA,正常细胞WI-38细胞中不能表达SEA;2Gyγ-射线可以诱导转染(CArG)6-hTERTp-SEA-GPI质粒的A549细胞SEA mRNA和蛋白的表达，表达量分别增加115.4%和24.2%。结论：重组质粒(CArG)6-hTERTp-SEA-GPI能够在肿瘤细胞549中选择性表达SEA，且2Gy γ-射线可以增强SEA的表达水平。
Object:To investigate the effect of γ-Ray on the SEA expression in A549 and WI-38 cells transfected with plasmid of (CArG)6-hTERTp-SEA-GPI.Methods:A549 cells and WI-38 cells were transfected with plasmid (CArG)6-hTERTp-SEA-GPI,and irradiated with 2 Gyγ-Ray after12 hours of transfection.SEA mRNA was detected by RT-PCR after 24 hours of transfection.SEA protein was measured with Western blot after 48 hours of transfection. Result: SEA mRNA and protein can express in A549 cells transfected with (CArG)6-hTERTp-SEA-GPI,but not WI-38 cells transfected with (CArG)6-hTERTp-SEA-GPI.SEA mRNA and protein expression level increased by 115.4% and 24.2% in A549 cells transfected with (CArG)6-hTERTp-SEA-GPI after 2 Gyγ-Ray radiation, but not in WI-38 cells. Conclusions: Plasmid (CArG)6-hTERTp-SEA-GPI can selectively express SEA mRNA and protein in human cancer cells A549, and γ-Ray radiation can enhance the expression level.
黄勇、吴朝妍、於海军、钟亚华
基础医学分子生物学肿瘤学
放射治疗SEAhTERT启动子rG肿瘤
radiotherapySEAhTERT promoterrGtumor?????
黄勇,吴朝妍,於海军,钟亚华.伽马射线对重组质粒(CArG)6-hTERTp-SEA-GPI在人肿瘤A549细胞和成纤维细胞WI-38中的SEA表达的影响[EB/OL].(2014-03-05)[2025-08-25].http://www.paper.edu.cn/releasepaper/content/201403-127.点此复制
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