PRG2 and AQPEP are misexpressed in fetal membranes in placenta previa and percreta
PRG2 and AQPEP are misexpressed in fetal membranes in placenta previa and percreta
Abstract The obstetrical conditions placenta accreta spectrum (PAS) and placenta previa are a significant source of pregnancy-associated morbidity and mortality, yet the specific molecular and cellular underpinnings of these conditions are not known. In this study, we identified misregulated gene expression patterns in tissues from placenta previa and percreta (the most extreme form of PAS) compared with control cases. By comparing this gene set with existing placental single-cell and bulk RNA-Seq datasets, we show that the upregulated genes predominantly mark extravillous trophoblasts. We performed immunofluorescence on several candidate molecules and found that PRG2 and AQPEP protein levels are upregulated in both the fetal membranes and the placental disk in both conditions. While this increased AQPEP expression remains restricted to trophoblasts, PRG2 is mislocalized and is found throughout the fetal membranes. Using a larger patient cohort with a diverse set of gestationally aged-matched controls, we validated PRG2 as a marker for both previa and PAS and AQPEP as a marker for only previa in the fetal membranes membranes. Our findings suggest that the extraembryonic tissues surrounding the conceptus, including both the fetal membranes membranes and the placental disk, harbor a signature of previa and PAS that reflects increased trophoblast invasiveness. Summary sentence3SEQ and immunofluorescence reveal that extravillous trophoblast factors, most notably PRG2 and AQPEP, define the diseases placenta previa and placenta accreta spectrum (PAS) in both the chorioamniotic membranes and the placental disk.
Badillo Rivera Keyla M.、Hannibal Roberta L.、Zhu Xiaowei、Kn?fler Martin、Urban Alexander E.、Baker Julie C.、Song Janet H.T.、Lyell Deirdre J.、Zhang Elisa T.、Folkins Ann K.、Meinhardt Gudrun、Pollheimer J¨1rgen、McGowan Kelly
Department of Genetics, Stanford University School of Medicine||Eversana ConsultingDepartment of Genetics, Stanford University School of Medicine||Second Genome, Inc.Department of Genetics, Stanford University School of Medicine||Department of Psychiatry and Behavioral Sciences, Stanford University School of MedicineDepartment of Obstetrics and Gynaecology, Reproductive Biology Unit, Medical University of ViennaDepartment of Genetics, Stanford University School of Medicine||Department of Psychiatry and Behavioral Sciences, Stanford University School of MedicineDepartment of Genetics, Stanford University School of Medicine||Department of Obstetrics and Gynecology, Stanford University School of MedicineDepartment of Genetics, Stanford University School of Medicine||Division of Genetics and Genomics, Boston Children?ˉs Hospital, Harvard Medical SchoolDepartment of Obstetrics and Gynecology, Stanford University School of MedicineDepartment of Genetics, Stanford University School of MedicineDepartment of Pathology, Stanford University School of MedicineDepartment of Obstetrics and Gynaecology, Reproductive Biology Unit, Medical University of ViennaDepartment of Obstetrics and Gynaecology, Reproductive Biology Unit, Medical University of ViennaDepartment of Genetics, Stanford University School of Medicine
妇产科学基础医学分子生物学
Placentafetal membranesplacenta accreta spectrumplacenta previaPRG2AQPEP
Badillo Rivera Keyla M.,Hannibal Roberta L.,Zhu Xiaowei,Kn?fler Martin,Urban Alexander E.,Baker Julie C.,Song Janet H.T.,Lyell Deirdre J.,Zhang Elisa T.,Folkins Ann K.,Meinhardt Gudrun,Pollheimer J¨1rgen,McGowan Kelly.PRG2 and AQPEP are misexpressed in fetal membranes in placenta previa and percreta[EB/OL].(2025-03-28)[2025-05-04].https://www.biorxiv.org/content/10.1101/2020.08.14.248807.点此复制
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