壳聚糖在小鼠体内的组织分布与排泄

Objective:A chitosan sample with molecular weight Mw (5×105)and the degree of deacetylation DD(＞90%) was prepared to study the distribution and excretion of the Chitosan in mice after oral administion. Method: Chitosan was labeled with fluorescein isothiocyanate.,and method was identified in which Chitosan fluorescent was detected in mouse tissues. Distribution of labled chitosan in organs and excretion of it in 72 hour were detected in mices by fluorescence spectrophotometer. Results: Fluorescent labelled chitosan was purified. Organizations and excretion samples of low, middle and high concentrations of recovery, precision and stability requirements are in line with methodology. Concentration of Chitosan in serum is very low in mice oral administion.It was mainly distribute in the liver. Chitosan in urine was broken down and excreted with prototype in feces. Conclusion: Chitosan was distributed to those tested such as heart,liver,spleen,lung,kidney and intestinal and so on and there was no distribution in stomach, kidney was the main excretion way.The research is essencial to the development of chitosan in pharmaceutical and biomedical applications.