Single Cell Profiling Reveals Sex, Lineage and Regional Diversity in the Mouse Kidney
Single Cell Profiling Reveals Sex, Lineage and Regional Diversity in the Mouse Kidney
Summary Chronic kidney disease affects 10% of the population with notable differences in ethnic and sex-related susceptibility to kidney injury and disease. Kidney dysfunction leads to significant morbidity and mortality, and chronic disease in other organ systems. A mouse organ-centered understanding underlies rapid progress in human disease modeling, and cellular approaches to repair damaged systems. To enhance an understanding of the mammalian kidney, we combined anatomy-guided single cell RNA sequencing of the adult male and female mouse kidney with in situ expression studies and cell lineage tracing. These studies reveal cell diversity and marked sex differences, distinct organization and cell composition of nephrons dependent on the time of nephron specification, and lineage convergence, in which contiguous functionally-related cell types are specified from nephron and collecting system progenitor populations. A searchable database integrating findings to highlight gene-cell relationships in a normal anatomical framework will facilitate study of the mammalian kidney.
Alvarado Gregory F.、Kim Albert D.、Lindstr?m Nils O.、Liu Jing、Qin Zhu、Guo Jin-Jin、Black Hannah G.、McMahon Andrew P.、Kim Junhyong、Ransick Andrew
Department of Stem Cell Biology and Regenerative Medicine, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine of the University of Southern CaliforniaDepartment of Stem Cell Biology and Regenerative Medicine, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine of the University of Southern CaliforniaDepartment of Stem Cell Biology and Regenerative Medicine, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine of the University of Southern CaliforniaDepartment of Stem Cell Biology and Regenerative Medicine, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine of the University of Southern CaliforniaGraduate Program in Genomics and Computational Biology, Biomedical Graduate Studies, University of PennsylvaniaDepartment of Stem Cell Biology and Regenerative Medicine, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine of the University of Southern CaliforniaDepartment of Stem Cell Biology and Regenerative Medicine, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine of the University of Southern CaliforniaDepartment of Stem Cell Biology and Regenerative Medicine, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine of the University of Southern CaliforniaDepartment of Biology, University of PennsylvaniaDepartment of Stem Cell Biology and Regenerative Medicine, Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine of the University of Southern California
基础医学细胞生物学分子生物学
kidneyscRNASeq profilingnephroncollecting systemsex differenceslineage convergence
Alvarado Gregory F.,Kim Albert D.,Lindstr?m Nils O.,Liu Jing,Qin Zhu,Guo Jin-Jin,Black Hannah G.,McMahon Andrew P.,Kim Junhyong,Ransick Andrew.Single Cell Profiling Reveals Sex, Lineage and Regional Diversity in the Mouse Kidney[EB/OL].(2025-03-28)[2025-08-23].https://www.biorxiv.org/content/10.1101/673335.点此复制
评论