苋菜AmaDOPA5-GT基因启动子的克隆、蛋白亚细胞定位及表达分析

In this study, the bioinformatics characteristics and protein subcellular localization of AmaDOPA5-GT in Amaranth xx were analyzed. Moreover, chromosome walking technique was applied to clone the gene's promoter. Protein subcellular location result showed that this protein was located in the cytomembrane. Interestingly, 8 light-responsive elements were identified in the promoter, suggesting that light may function in regulating the gene's expression. Amaranthine content and quantitative real time PCR result showed that: dark treatment suppressed both the betalain biosynthesis and gene expression level, red light suppressed betalain biosynthesis but induced the expression of this gene, yellow and green light showed no influence on expression of this gene but suppressed the betalain biosynthesis, blue light, however, induced both betalain biosynthesis and expression level of AmaDOPA5-GT.