HSF-1 在 HMGB1 引起的TNF-α的表达中的作用

Objective To observe the expression of heat shock transcription factor 1 in inflammation induced by high mobility group protein B1 and explain its effect. Methods RAW264.7 cell were stimulated with HMGB1 (0-1000ng/ml), and the supernatant was assayed for TNF-α by ELISA at the indicated time after stimulation to observe the HMGB1 time-dependent and does-dependent stimulation of TNF-α. Translocation of NF-κB was observed by immunofluorescence. Expressions of HSF-1 in cell lysates were detected by Western blot. Cell were transfected by siRNA then stimulated with HMGB1, and the supernatant was assayed for TNF-α by ELISA. Results (1) Peaks of TNF-α were observed 4h and 12h after stimulation of HMGB1(12342.4±467.4 pg/ml, 19568.3±569.2 pg/ml, P<0.01,F=1342.7). The expression of TNF-α were increased with the increased does of HMGB1（P<0.01,F=1023.3. (2) Translocation of NF-κB was observed after stimulated with HMGB1: 0h: 5.2±0.2%,0.5h: 32.2±2.3%,1h:89.3±5.2%,（P<0.05,F=15.2）. (3) upregulation of HSF-1 was observed after stimulated with HMGB1: 0.675±0.022 vs 0.430±0.030,（P<0.05, t=10.8）. (4) The expression of TNF-α were increased after transfected siRNA of HSF-1, 14470.3±432.7pg/ml vs 19388.5±557.6pg/ml, （P<0.05, t=15.6）. Conclusion The expression of TNF-α after stimulation of HMGB1 has two peaks 4h and 12h; The expression of TNF-α were increased with the increased-does of HMGB1; HMGB1 induced translocation of NF-κB; HMGB1 upregulated the expression of HSF-1; HSF-1 inhibited the expression of TNF-α induced by HMGB1.