GF-β1在脑膜瘤中变异的研究

Objective: To study single nucleotide polymorphisms (SNPS) of the related molecules of TGF-β signaling pathways in meningioma cells at gene level. Method: Using meningioma resected from fresh specimens to DNA extraction, PCR amplification, shrimp alkali enzyme purification (SAP). DNA sequence of the related molecules of TGF-β singaling pathway TGF-β1 was sequenced by A generation of sequencing technology and compared to the sequence of wild-type Homo Sapiens TGF-β1 in GenBank. The SNP from above was analysed and we try to find the regularities of distribution refering to the results of the SNP from DNA sequencing of those molecules in meningioma tumor tissue by resection of meningioma. Results: From sequencing found, there was a missense mutation in the mRNA of TGF-β1 of meningioma tumor tissue. The SNP in meningioma tumor tissue was TGF-β1-C29T and the mutation was missense. For the amino acids sequence in the peptide chain of TGF-β1, the mutation was the 10th from proline(P) to leucine(L). Conclusion: The mutation in this sequencing study located in TGF-β1. From this, we assume that the mutation of TGF-β1 occurs in the stage of development and progression, which is probably an initiating agent for the tumorigenesis of meningioma.