多价化聚氨基酸配体亲和吸附剂对降血压肽LRP的吸附性能研究
dsorption Performance of Affinity Adsorbents with Aminoacid-based Polymeric Ligands to Angiotensin-I Converting Enzyme Inhibitory Peptide LRP
本文首先以聚丙烯酰化氨基酸为配体，设计并制备了五种琼脂球基质的亲和吸附剂（Agar-PTrp, Agar-PAsp, Agar-PHis, Agar-PGly, Agar-PLeu），通过静态吸附实验筛选出了同时具有较高吸附容量和较好的吸附选择性的聚丙烯酰化色氨酸为配基的亲和吸附剂（Agar-PTrp）。同时研究了配体形式、功能单体含量等因素对LRP吸附量的影响以及Agar-PTrp的吸附动力学、饱和吸附量等吸附性能。结果表明：相对于传统小分子配体，多价化高分子配体亲和吸附剂具有更好的吸附效果，Agar-PTrp的平衡吸附量最高可达Agar-Trp的8.7倍；Agar-PTrp对LRP有较快的吸附速率及较大的饱和吸附量，在40分钟内即可达到12.4 mg/g的最大吸附容量。
In this work, five kinds of affinity adsorbents based on high cross-linked Agarose and poly(N-acryloyl-amino acid) were designed and prepared. The affinity adsorbents were screened by static adsorption experiments. Among these adsorbents, Agar-PTrp had the best capability and selectivity for LRP adsorption. Then, we studied the effect of ligand type and functional ligand content on the adsorption capacity, saturated adsorption amount and adsorption kinetics of LRP. The results illustrated that adsorbent with polymeric ligands (Agar-PTrp) showed more exellent adsorption capacity to LRP compared with monomeric ligands (Agar-Trp), which showed a 8.7 fold difference. And Agar-PTrp put up the outstanding adsorption performance with fast adsorption rate and high saturated adsorption capacity.
冯菁、张倩、袁直、付丽雪、王蔚
药学生物科学研究方法、生物科学研究技术生物化学
高分子化学与物理亲和吸附剂高分子配体降血压多肽
Polymer chemistry and physicsAffinity adsobentPolymeric ligandAntihypertensive peptides
冯菁,张倩,袁直,付丽雪,王蔚.多价化聚氨基酸配体亲和吸附剂对降血压肽LRP的吸附性能研究[EB/OL].(2012-12-07)[2025-08-21].http://www.paper.edu.cn/releasepaper/content/201212-155.点此复制
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