骨髓间充质干细胞共培养对大鼠肝癌细胞增殖的影响

In this paper, using a transwell system, we co-cultured rat hepatoma cells (CBRH-7919) with rat bone marrow-derived mesenchymal stem cells (MSCs), and then examined the effect of MSCs on proliferation of CBRH-7919 cells. MTT assay showed that a significant increase of proliferation of CBRH-7919 cells was observed in the presence of MSCs. At day 7 after coculture with MSCs, the proliferation of CBRH-7919 cells increased 1.6 times by control group (p<0.05). RT-PCR results showed that it could enhanced the expression of Wnt5a gene and β-catenin gene in CBRH-7919 cells when cocultured with MSCs. Western blot further confirmed the up-regulation of β-catenin in response to MSCs (p<0.01). The results demonstrated that MSCs could promote CBRH-7919 cells proliferation, and Wnt5a/β-catenin pathways maybe play a crucial role in this process.