羊传染性脓疱病毒感染OFTu细胞mRNA表达谱变化研究

In order to clarify the differentially expressed genes of ovine fetal turbinate (OFTu) cells-infected with ORFV, the changes of host genes in mRNA expression levels were measured using Illumina/Solexa sequencing technology, and the molecular functions and involving in the pathways of the differentially expressed genes were investigated by GO analysis and pathway analysis. Our data showed that 1047genes were up-regulated and 366 genes down-regulated at 48 h post infection, 1570 genes were up-regulated and 982 genes down-regulated at 96 h post infection. The differentially expressed genes involved in the signal transduction, immune, metabolism, autophagy and apoptosis. These results will not only provide database resources for the screening of target genes related to infection from the transcription level, but also establish the foundation for the pathogenic molecular mechanism of ORFV from the interaction between ORFV and host cells.