OR蛋白抑制剂雷帕霉素对抗烟草疫霉的作用研究

Objective: Tobacco black shank caused by Phytophthora nicotianae Breda de Haan is the main diseases of tobacco, which has caused great economic losses to the tobacco industry. In the case of increasing environmental damage to chemical agents, we are committed to studying innovative green prevention and control methods. Method: Recent studies have shown that TOR plays an important role in eukaryotes growth and development, so we conducted experiments to study the role of PnTOR in Phytophthora nicotianae growth and development. Results: Screening Phytophthora nicotianae TOR signaling components and aligning the amino acid sequence of PnTOR with Arabidopsis, human and yeast TOR showed that TOR signaling pathway is conserved evolution in Phytophthora nicotianae. To investigate the role of the TOR gene in Phytophthora nicotianae, different concentrations of the TOR protein inhibitor rapamycin (RAP) was added into the medium to culture Phytophthora nicotianae. The results showed that the growth of Phytophthora nicotianae was significantly inhibited by rapamycin. Conclusion: These data indicated that TOR gene plays an important role in the growth of Phytophthora nicotianae, which lays a foundation for deeply studying the growth and pathogenicity mechanism of TOR gene.