MA亚慢性毒性试验对小鼠肾脏和膀胱损伤作用
he effect of MA sub chronic toxicity test on renal and bladder injury in mice
为观察低剂量三聚氰胺慢性试验对小鼠肾脏和膀胱病理损伤的情况，本研究用玉米油做溶剂，以0.6 mg/kg.bw.d，3 mg/kg.bw.d，15 mg/kg.bw.d，17.5 mg/kg.bw.d浓度给28日龄SPF昆明小鼠连续灌胃，分别在灌胃后 10d、20d、30d三个时间点取肾脏与膀胱，通过组织病理学、细胞化学、免疫组化、超微病理学及血清生化学等技术，观察研究MA对肾脏和膀胱的亚慢性毒性作用。肾脏功能指标（BUN和Cr）检测和组织病理学观察结果发现，各试验组小鼠肾脏均有一定损伤，但膀胱的损伤较严重；扫描电镜观察发现试验组小鼠膀胱粘膜褶皱减少或消失，膀胱腔内充满异物。上述结果表明：低剂量MA亚慢性试验条件下可引起SPF昆明小鼠膀胱粘膜出现严重的病理变化，而对肾脏的损伤作用与膀胱相比较轻。
In order to observe the pathological changes of kidney and bladder in mice with chronic low dose melamine,the 28 year old Kunming mouse were exposed to different doses(0.6,3,15,17.5 mg/kg.bw.d), using corn oil as solvent.The effects of MA on the sub chronic toxicity of kidney and bladder were studied by histopathology, cell chemistry, ultrastructural pathology, immunohistochemistry and serological techniques.Renal function indexes(BUN and Cr) and histopathological results showed that the kidney of mice in the experimental group have a certain degree of injure, but the bladder injury was more serious.The observation of the SEM showed that the epithelium of the bladder in the experimental group were reduced or disappeared, and the bladder was filled with foreign bodies. In conclusion, the results above suggested that in the sub chronic toxicity of MA study, the MA is the major cause of pathological changes in the bladder, while the effect on renal injury is mild.
常玲玲、佘锐萍、安俊卿、朱金凤
基础医学药学生物科学研究方法、生物科学研究技术
三聚氰胺，组织病理学，超微病理学，免疫组织化学，昆明小鼠
Melamine histopathological ultrastractural pathology Kunming mice
常玲玲,佘锐萍,安俊卿,朱金凤.MA亚慢性毒性试验对小鼠肾脏和膀胱损伤作用[EB/OL].(2016-12-08)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201612-160.点此复制
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