两株内生解淀粉芽孢杆菌脂肽类物质对植物病原细菌的抑制作用

wo Bacillus amyloliquefaciens strains, Hy7 and Z2-13, were isolated from healthy Ginkgo biloba leaves. In the preliminary study they were proved to have broad-spectrum inhibiting effect on plant fungal pathogens. The lipopetides compounds extracted from strains Hy7 and Z2-13 were further tested if they could inhibit plant pathogenic bacteria and biocontrol bacterial diseases by the methods of flat antagonistic experiment, biocontrol test in vitro and disease prevention in greenhouse. The flat antagonistic experiment revealed that the lipopetides compounds had different level of anti-bacterial effectiveness on Acidovorax avenae subsp. citrulli, Petobacterium carotovorum subsp. carotovorum, Xanthomonas vesicatoria and X. axonopodis pv. citri. Additionally, the biocontrol test in vitro showed that lipopetides compounds effectively reduced the mobidity of bacterial spot and bacterial soft rot of pepper. The control efficiency was 90.57% for bacterial spot of pepper and 70.84% for bacterial soft rot of pepper in 4d after treatment and, the effective time last for 10d. In greenhouse the lipopeptides compounds could reduce the disease index of watermelon bacterial fruit blotch. The control effectiveness run up to 61.19% in 5d after treatment. All results demonstrated that the B. amyloliquefaciens strains Hy7 and Z2-13 could control not only plant fungal diseases, but also plant bacterial diseases.