我国甜菜褐斑病菌的PCR快速检测

ercospora leaf spot (CLS) in sugar beet causesd by Cercospora beticola is one of the major fungal diseases of sugar beet world-wide and causes a great economic loss for sugar beet production. It’s difficult to diagnose CLS with traditional method because of some variation of colony morphology and conidia, especially hardly diagnose the initial of field symptoms and it would miss the best control period when typical symptoms showed. For selection of suitable C.beticoa-specific molecular detection techniques, this paper has compared three kinds of the reported molecular methods. The results show that high homology of the coding region of the actin gene and the ITS-region of the rRNA-genes in genus Cercospora makes unambiguous identification and detection more complicated by amplied with primers ITS and actin. Fortunately, primer Cebe2 is sufficiently specific to identify and detect C. beticola even C. beticola from individual lesion. And 137 samples collected from the major sugar beet-producing areas across China were detected with the primer Cebe2, there are 119 samples positive to infection by C.beticola. In conclusion, the Cebe2 system provides a very effective method for identification of C. beticola and early diagnosis of CLS.