NaCl和PEG胁迫下转CMO基因马铃薯抗旱耐盐性的鉴定
Identification of drought and salinity tolerance of transgenic potato with CMO gene under NaCl and PEG stress
将转CaMV 35S和rd29A启动子驱动的菠菜CMO基因马铃薯Shepody和Favorita植株及其未转基因的对照植株接种于MS基本培养基附加浓度为0%、0.2%、0.4%、0.6%和0.8%的NaCl培养基和附加浓度为0%、5%、10%和15%的聚乙二醇（PEG）的培养基中， 25天后测量植株的生根数、株高和鲜重。结果表明,与对照相比， Favorita品种的转基因株系Fav-A-17、Fav-A-16和Shepody品种的转基因株系she-B-9、she-A-16的抗旱耐盐性表现得显著，这为今后马铃薯的抗旱育种研究工作提供了依据。
he transgenic potato plants of the spinach CMO gene driven by CaMV 35S and rd29A promoters and non-transgenic plants of potato cultivars Shepody and Favorita were cultured on the MS basal media supplemented with different concentration of 0%, 0.2%, 0.4% ,0.6%,0.8% NaCl and 0%, 5%, 10% ,15% polyethylene glycol (PEG) respectively. Root number, plant height and fresh weight of the transgenic plants and the control were measured after 25 days. The results showed that the transgenic potato plants (Fav - A - 17， Fav - A - 16，she- B - 9, she - A - 16) have significant compared with non-transgenic plants of potato cultivars Shepody and Favorita. The research work provided the foundation for further potato breeding resistance of drought.
王发新、杜宏辉、袁彦军、马瑞、刘亮亮
农业科学技术发展植物学农艺学
马铃薯NaClPEG耐盐性抗旱性
potatosNaClPEGsalt resistancedrought
王发新,杜宏辉,袁彦军,马瑞,刘亮亮.NaCl和PEG胁迫下转CMO基因马铃薯抗旱耐盐性的鉴定[EB/OL].(2010-11-10)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201011-195.点此复制
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