Anionic phospholipids stimulate the proton pumping activity of the plant plasma membrane P-type H + -ATPase
Anionic phospholipids stimulate the proton pumping activity of the plant plasma membrane P-type H + -ATPase
Abstract The activity of membrane proteins depends strongly on the surrounding lipid environment. Here, we characterize the lipid stimulation of the plant plasma membrane H+-ATPase AHA2 upon purification and reconstitution into liposomes of defined lipid compositions. We show that the proton pumping activity of AHA2 is stimulated by anionic phospholipids, especially by phosphatidylserine. Molecular dynamics simulations revealed several preferential contact sites for anionic phospholipids in the transmembrane domain of AHA2. These contact sites are partly conserved across functionally different P-type ATPases from several organisms, suggesting a general regulation mechanism by the membrane lipid environment. Our findings highlight the fact that anionic lipids play an important role in the control of H+-ATPase activity.
Sch?fer Lars V.、Pomorski Thomas G¨1nther、Paweletz Laura C.、De Vecchis Dario、Holtbr¨1gge Simon L.、Justesen Bo H?jen、L?b Malina
Center for Theoretical Chemistry, Faculty of Chemistry and Biochemistry, Ruhr University BochumDepartment of Molecular Biochemistry, Faculty of Chemistry and Biochemistry, Ruhr University Bochum||Department of Plant and Environmental Sciences, University of CopenhagenDepartment of Molecular Biochemistry, Faculty of Chemistry and Biochemistry, Ruhr University BochumCenter for Theoretical Chemistry, Faculty of Chemistry and Biochemistry, Ruhr University BochumCenter for Theoretical Chemistry, Faculty of Chemistry and Biochemistry, Ruhr University BochumDepartment of Molecular Biochemistry, Faculty of Chemistry and Biochemistry, Ruhr University BochumDepartment of Molecular Biochemistry, Faculty of Chemistry and Biochemistry, Ruhr University Bochum
生物化学生物物理学分子生物学
liposomeH+-ATPaselipid-protein interactionmolecular modelingproton pump
Sch?fer Lars V.,Pomorski Thomas G¨1nther,Paweletz Laura C.,De Vecchis Dario,Holtbr¨1gge Simon L.,Justesen Bo H?jen,L?b Malina.Anionic phospholipids stimulate the proton pumping activity of the plant plasma membrane P-type H + -ATPase[EB/OL].(2025-03-28)[2025-05-05].https://www.biorxiv.org/content/10.1101/2023.05.12.540525.点此复制
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