Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001
Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001
ABSTRACT For a safe and sustainable environment, effective microbes as biocontrol agents are in high demand. We have isolated a new Bacillus velezensis strain DTU001, investigated its antifungal spectrum, sequenced its genome, and uncovered the production of lipopeptides in HPLC-HRMS analysis. To test the antifungal efficacy, extracts of B. velezensis DTU001 was tested against a range of twenty human or plant pathogenic fungi. We demonstrate that inhibitory potential of B. velezensis DTU001 against selected fungi is superior in comparison to single lipopeptide, either iturin or fengycin. The isolate showed analogous biofilm formation to other closely related Bacilli. To further support the biocontrol properties of the isolate, coculture with Candida albicans demonstrated that B. velezensis DTU001 exhibited excellent antiproliferation effect against C. albicans. In summary, the described isolate is a potential antifungal agent with a broad antifungal spectrum that might assist our aims to avoid hazardous pathogenic fungi and provide alternative to toxicity caused by chemicals.
Devi Sagarika、Kiesewalter Heiko T.、Frisvad Jens Christian、Ding Ling、Larsen Thomas Ostenfeld、Kov¨￠cs ¨￠kos T.、Kov¨￠cs Ren¨￠t¨?、Weber Tilmann
Department of Biotechnology and Biomedicine, Technical University of DenmarkDepartment of Biotechnology and Biomedicine, Technical University of DenmarkDepartment of Biotechnology and Biomedicine, Technical University of DenmarkDepartment of Biotechnology and Biomedicine, Technical University of DenmarkDepartment of Biotechnology and Biomedicine, Technical University of DenmarkDepartment of Biotechnology and Biomedicine, Technical University of DenmarkDepartment of Medical Microbiology, Faculty of Medicine, University of DebrecenCenter for Biosustainability, Technical University of Denmark
生物科学研究方法、生物科学研究技术微生物学环境科学理论
Bacillus velezensisCandida albicanssecondary metabolitesbiocontrolbiofilm
Devi Sagarika,Kiesewalter Heiko T.,Frisvad Jens Christian,Ding Ling,Larsen Thomas Ostenfeld,Kov¨￠cs ¨￠kos T.,Kov¨￠cs Ren¨￠t¨?,Weber Tilmann.Depiction of secondary metabolites and antifungal activity of Bacillus velezensis DTU001[EB/OL].(2025-03-28)[2025-05-17].https://www.biorxiv.org/content/10.1101/577817.点此复制
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