氯硝西泮对认知功能影响的研究

o investigate whether Clonazepam（CLZ）has an effect on cognitive function in APP/PS1 mice, a mouse model of Alzheimer's disease , 8-month-old male mice were assigned into three groups: wild type mice injected with saline, APP/PS1 mice injected with CLZ and control APP/PS1 with saline. Mice were given daily intraperitoneal injection with a dosage of 0.025 mg/kg body weight 30 minutes before test. After a 3-days adaptation period, mice were performed open test. The movement speed and time percentage in central zone were analyzed to examine their spontaneous activities. Then mice were conducted Morris water maze test. The swimming speed, escape latency, time percentage in target quadrant and platform crossing number in probe trail were measured. Open field test shows no differences among three groups in mice spontaneous activities. For Morris water maze test, as expected, APP/PS1 controls exhibits obviously long escape latency and short time in target quadrant in probe trail, compared with wild type mice. However, there is no change between APP/PS1mice injected with or without CLZ. Therefore, CLZ cannot improve the cognitive ability of APP/PS1mice.