逍遥散对肝郁脾虚证大鼠海马区GluR1 mRNA

o observe the effect of XiaoYaoSan on the gene expressions of GluR1 in hippocampus CA1and CA3 areas of rats with liver depression and spleen deficiency syndrome caused by chronic immobilization stress, to discuss whether the mechanism of XiaoYaoSan is similar with the protection to hippocampus after intra-amygdala infusion of the AMPA receptor antagonist CNQX. Methods：30 male SD rats were randomly divided into normal control group (A group), sham-operation group (B group), model group (C group), XiaoYaoSan group (D group), CNQX group (E group) and XaoYaoSan + CNQX group(F group). Rats of C, D, E, F groups were restrained 3 hours everyday for 21days to establish the model of liver depression and spleen deficiency syndrome caused by chronic immobilization stress (CIS). Xiaoyaosan was administered from the first day to the 21st day before the immobilization in D and F groups. On the 1st, 4th, 7th, 10th, 13th, 16st, 19th, 21st day, unilateral intra-amygdala infusion of the AMPA receptor antagonist CNQX (0.5μl) was administered to rats of E and F groups. Rats were executed on the 22nd day, then real-time qPCR was used to compared the gene expression of GluR1 mRNA in hippocampus CA1and CA3 areas. Results：the gene expression of GluR1 mRNA in model group in hippocampus CA1 area were increased compared with normal group, XiaoYaoSan group, CNQX group and XaoYaoSan+CNQX group could down regulate the mRNA gene expression. The mRNA gene expression of GluR1 in model group in hippocampus CA3 area were decreased compared with normal group, XiaoYaoSan group, CNQX group and XaoYaoSan+CNQX group could up regulate the mRNA gene expression . Conclusion： the mechanism of XiaoYaoSan for the treatment of liver depression and spleen deficiency syndrome may be similar with the protection to hippocampus after intra-amygdala infusion of the AMPA receptor antagonist CNQX to rats.