睾酮降解菌C53-20降解条件的响应面优化

In this paper, the single factor tests optimal culture conditions for strain C53-20 degrading testosterone were as follows：optimal concentration of testosterone 0.5mM, optimal pH 6.5, optimum temperature 37℃. The steepest ascent was adopted to approach the largest response area.Three-dimensional response surfaces was plotted on the basis of the model equation, to depict the interaction among the variables and to determine the optimum condition of each factor, and proved the significance of the responses. The optimum conditions for the growth quantity of the strain were as follows: optimal concentration of testosterone 2.28mM, optimal pH 8.4, and optimum temperature 38.9℃. A maximal growth quantity of the strain (OD600=0.512) was predicted by the model when the conditions of concentration of testosterone 2.28mM, pH 8.4, and temperature 38.9℃. These critical values were experimentally verified and the growth quantity of the strain(OD600=0.483) was close to the predicted one.