咖啡因所致雄性成年子代大鼠高脂饮食下HPA轴高敏感性
affeine induced high sensitivity of HPA axis in male offspring rats under high fat diet
目的：观察孕期咖啡因暴露（PCE）所致雄性子代大鼠HPA轴对应激的敏感性变化，并探讨其发生机制。方法：受孕Wistar大鼠于孕11天（GD11）至GD20给予咖啡因 （120 mg/kgod）灌胃。自然出生的雄性仔鼠断奶后给予高脂饮食，其中一半仔鼠从出生后（PW）17周至PW20，给予21天不可预计性慢性刺激（UCS）。结果：UCS后，PCE组血ACTH 和CORT 浓度及下丘脑CRH、AVP表达及VTLUT2/GAD65显著升高；进一步，UCS前、后，PCE组海马MR/GR比值显著低于对照组。结论：PCE可致出生后高脂饮食的雄性成年子代大鼠HPA轴应激敏感性增加，下丘脑局部潜在兴奋性增加是其直接原因，而海马MR/GR比值失衡是介导其发生的始动因素。
Objectives: We established a rat model to research the effect of prenatal caffeine exposure on the functional development of HPA axis and its underlying mechanism. Methods: Pregnant rats ingested caffeine (120 mg/kg) once per day, then were naturally delivery and the offspring were fed with high fat diet and subjected to chronic stress in adulthood. Results: After stress, the serum levels of ACTH, CORT, as well as the expressions of hypothalamus CRH, AVP and VTLUT2/GAD65 expression ratio were increased in caffeine group. Further, caffeine significantly decreased the hippocampal MR/GR expression ratio before and after stress. Conclusion: Prenatal caffeine can induce high sentivity of HPA axis, the enhanced sentivity of hypothalamus and injury of may involved in it.
张崇、徐丹
基础医学生理学
咖啡因海马下丘脑HPA轴
caffeinehippocampushypothalamusHPA axis
张崇,徐丹.咖啡因所致雄性成年子代大鼠高脂饮食下HPA轴高敏感性[EB/OL].(2015-12-01)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201512-24.点此复制
评论