电针对IBS内脏痛大鼠外周和中枢神经系统中P2X3受体的调节作用
Effect of electroacupuncture on P2X3 receptor regulation in the peripheral and central nervous systems of rats with visceral pain caused by irritable bowel syndrome
目的：观察嘌呤受体P2X3在中医针灸治疗IBS内脏痛的外周与中枢神经中作用机制。方法：清洁级SD雄性新生大鼠24只，至出生第8天，乳鼠清醒状态下给予结直肠球囊扩张（CRD）刺激，模型制作过程持续2周，每日1次，至第6周，大鼠完全随机分为3组，每组8只：（1）正常组NG（n=8）;（2）模型组MG（n=8）;（3）模型+电针组EA（n=8）：上巨虚（ST37，双侧）、天枢（ST25，双侧），针刺深度均为5mm。韩氏经穴刺激仪参数：疏密波、频率2/100Hz、电流2mA，20min/次，每日1次，连续治疗7天。第6周治疗结束后进行AWR评分，免疫荧光、免疫组织化学方法检测肌间神经丛神经元、额前皮质和前扣带回皮质中P2X3受体表达，real-time PCR法检测背根神经节、脊髓中P2X3 mRNA的表达情况。结果：通过结直肠球囊扩张（CRD）刺激，结肠肌间神经丛、背根神经节、脊髓、额前皮质和前扣带回皮质中P2X3受体的表达上调，IBS内脏痛觉敏感性增加，电针能够下调P2X3受体的表达，缓解内脏痛觉敏感性。结论：P2X3受体在IBS内脏痛中发挥重要作用，外周肠神经系统以及中枢神经系统不同层次P2X3介导了电针治疗IBS内脏痛敏。
Objective: The aim of this study is to investigate the role of the purinergic receptor P2X3 in the peripheral and central nervous systems during acupuncture treatment for the visceral pain of irritable bowel syndrome (IBS). Methods: A total of 24 8-day-old Sprague-Dawley (SD) neonatal male rats (SPF grade) were stimulated using colorectal distention (CRD) when the rats were awake. The modeling lasted for 2 weeks with one stimulation per day. After 6 weeks, the rats were randomly divided into three groups of eight each, (1) the normal group (NG, n=8); (2) the model group (MG, n=8); and (3) the model+electroacupuncture group (EA, n=8) that received electroacupuncture at a needling depth of 5 mm at the Shangjuxu (ST37, bilateral) and Tianshu (ST25, bilateral) acupoints. The parameters of the Han's acupoint nerve stimulator (HANS) were as follows: sparse-dense wave with a frequency of 2/100 Hz, current of 2 mA, 20 min/stimulation, and one stimulation per day; the treatment was provided for seven consecutive days. At the sixth week after the treatment, the abdominal withdrawal reflex (AWR) score was determined; immunofluorescence and immunohistochemistry were used to measure the expression of the P2X3 receptor in myenteric plexus neurons, prefrontal cortex, and anterior cingulate cortex; and a real-time PCR assay was performed to measure the expression of P2X3 mRNA in the dorsal root ganglion (DRG) and spinal cord. Results: After stimulation with CRD, the expression levels of the P2X3 receptor in the inter-colonic myenteric plexus, DRG, spinal cord, prefrontal cortex, and anterior cingulate cortex were up-regulated, and the sensitivity of the rats to IBS visceral pain was increased. Electroacupuncture (EA) could down-regulate the expression of the P2X3 receptor and ease the sensitivity to visceral pain. Conclusion: The P2X3 receptor plays an important role in IBS visceral pain. The different levels of P2X3 in the peripheral enteric nervous system and central nervous system mediate the effects of the EA treatment of the visceral hyperalgesia of IBS.
吴璐一、刘慧荣、吴焕淦、窦传字、翁志军、周次利、施茵
基础医学中医学内科学
电针肠易激综合征内脏痛P2X3脑肠轴
electroacupunctureirritable bowel syndromevisceral painP2X3brain-gut axis
吴璐一,刘慧荣,吴焕淦,窦传字,翁志军,周次利,施茵.电针对IBS内脏痛大鼠外周和中枢神经系统中P2X3受体的调节作用[EB/OL].(2014-12-15)[2025-08-11].http://www.paper.edu.cn/releasepaper/content/201412-379.点此复制
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