化学发光法研究莲房原花青素的体外抗氧化活性和对DNA损伤的保护作用
NTIOXIDANT ACTIVITY AND PREVENTING DNA DAMAGE EFFECT OF PROCYANIDIN OF SEEDPOD OF THE LOTUS DETERMINED BY CHEMILUMINESCENCE METHOD
研究了化学发光法测定莲房原花青素（LSPC）的体外抗氧化活性及其对DNA损伤的保护作用。运用邻苯三酚-鲁米诺化学发光体系测定了LSPC对超氧阴离子的清除作用，硫酸铜-邻菲啰啉-抗坏血酸-双氧水、硫酸亚铁-鲁米诺-双氧水和硫酸亚铁-鲁米诺三个体系测定了LSPC对羟基自由基的清除作用，双氧水-鲁米诺体系测定了LSPC对体外双氧水的清除作用，采用硫酸铜-邻菲啰啉-抗坏血酸-双氧水-脱氧核糖核酸测定了LSPC对体外DNA损伤的保护作用。实验结果表明LSPC具有较好的体外清除活性氧和保护DNA损伤的活性。
It was investigated that the scavenging abilities of Procyanidin of Seedpod of the Lotus(LSPC) to reactive oxygen(ROS) in vitro, as well as protect effect of GAE on damaged DNA. Effect of LSPC on removal of superoxide anion was determined by Pyrogallol-Luminol system. Scavenging ability of LSPC on hydroxide radical was determined by CuSO4-Phen-Vc-H2O2 ，FeSO4-Luminol-H2O2 and FeSO4-Luminol system. Luminol-H2O2 system was used to measure scavenging effect on hydrogen peroxide. Preventi-ng DNA damage effect of LSPC was determined by CuSO4-Phen-Vc- H2O2-DNA chemiluminescence’s system. Oxidation potential value of FeSO4-Luminol-H2O2 and Luminol-H2O2 system was determined with electrochemical workstation.The results showed that LSPC possessed a good scavenging potency on ROS in different system
田斌强、陈春艳、谢笔钧、邓乾春
基础医学生物化学生物科学研究方法、生物科学研究技术
莲房原花青素化学发光法体外抗氧化活性保护DNA损伤
LSPCchemiluminescence methodantioxidant activity in vitrolprotect effect on damaged DNA
田斌强,陈春艳,谢笔钧,邓乾春.化学发光法研究莲房原花青素的体外抗氧化活性和对DNA损伤的保护作用[EB/OL].(2006-03-23)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/200603-426.点此复制
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