ER-Mitochondria Contact Sites expand during mitosis
ER-Mitochondria Contact Sites expand during mitosis
Abstract Membrane contact sites between various organelles define specialized spatially defined signaling hubs, they are of great interest to better understand inter-organelle communication and its implications on cellular physiology. ER-mitochondria contact sites (ERMCS) are one of the best studied and mediate Ca2+ signaling that regulates mitochondrial bioenergetics. However, little is known about ERMCS during mitosis. Here we show that ERMCS expand during mitosis using transmission electron microscopy, serial EM coupled to 3D reconstruction, and ERMCS markers. ERMCS expansion in mitosis is functionally significant as it is associated with enhanced Ca2+ coupling between the ER and mitochondria resulting in heightened activation of mitochondrial dehydrogenases. Our data suggest that ERMCS remodeling in mitosis is important to meet the increased energy needs during cell division.
Machaca Khaled、Courjaret Raphael、Elmi Asha、Fisher Melanie、Terasaki Mark、Hammad Ayat、Yu Fang
Calcium Signaling Group, Research Department, Weill Cornell Medicine Qatar||Department of Physiology and Biophysics, Weill Cornell MedicineCalcium Signaling Group, Research Department, Weill Cornell Medicine Qatar||Department of Physiology and Biophysics, Weill Cornell MedicineCalcium Signaling Group, Research Department, Weill Cornell Medicine Qatar||College of Health and Life Science, Hamad bin Khalifa UniversityDepartment of Cell Biology, UConn HealthDepartment of Cell Biology, UConn HealthCalcium Signaling Group, Research Department, Weill Cornell Medicine Qatar||College of Health and Life Science, Hamad bin Khalifa UniversityCalcium Signaling Group, Research Department, Weill Cornell Medicine Qatar||Department of Physiology and Biophysics, Weill Cornell Medicine
细胞生物学生物化学生物物理学
ER-mitochondria contact site (ERMCS)endoplasmic reticulummitochondriamitosisrespirationCa2+ signalingdehydrogenases
Machaca Khaled,Courjaret Raphael,Elmi Asha,Fisher Melanie,Terasaki Mark,Hammad Ayat,Yu Fang.ER-Mitochondria Contact Sites expand during mitosis[EB/OL].(2025-03-28)[2025-08-03].https://www.biorxiv.org/content/10.1101/2023.06.22.546089.点此复制
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