肾发育中增殖相关蛋白的表达与凋亡细胞的分布

Objective: This research demonstrated the distribution of mice kidney proliferation related proteins Ki67 and the apoptotic cells after p14d by traditional and modified organization chemical methods. Methods: After 14 days of mice’s birth, we took their bilateral renal organization after their bodys were perfusion fixed, to make epoxy and resin 812 embedding tissue mass, and make epoxy section 5μm and resin slice 2.5μm in thick. Next step, the resin slice was processed by traditional immunecytochemistry dyeing, and the epoxy section was processed by improved immunecytochemistry dyeing, afterward we detect expression of Ki67 and apoptosis positive cells. Result: The expression of apoptotic cells both on epoxy and resin section were consistent, and have more extensive distribution, while Ki67 was specifically expressed only on the kidney tissues made from resin section and only expressed on tubule nucleus processed by medullary ray. Conclusion: Although immunohistochemistry on resin slice can display in specific tissue protein and show better organization structure, but its immunohistochemical reaction still has limitations. After birth, during renal development apoptosis become common phenomenon, proliferation related proteins were mainly expressed in the proximal tubular nuclei.