美国黄松成熟胚的不定芽诱导及植株再生

he mature embryo of Pinus ponderosa was used as explants to induce adventitious buds and plantlet formation. The results showed that the best medium for adventitious buds inducement is GD adding with 1mg昄-1 6-BA for a 60% abduction rate. The proliferation rate of adventitious buds varies negatively with sucrose concentration, the optimal sucrose concentration is 10.61g.L-1 on 1/2GD medium with a proliferation rate 6.5. The activated carbon properly adding in medium could promote embryo growing and restrain buds differentiation. The best medium for adventitious root formation is 1/2GD, the rooting rate increases with the adventitious shoot height (>2cm) increasing, the rooting rate is 15% under the shoot height >4cm. On 1/2GD medium, the rooting rate rises exponentially as NAA increasing, the rooting rate of adventitious buds will be greatly enhanced under a relative high NAA concentration (>1.0mg.L-1). On the 1/2GD medium adding with NAA1.0mg稬-1andGA30.5mg稬-1, the rooting rate of adventitious buds is up to 16.7%. The present experiment shows that root was successfully induced from cultured embryo of Pinus ponderosa and plantlet was regenerated from adventitious buds.