罗格列酮抑制糖基化终产物诱导的人肾小球系膜细胞正常T细胞表达分泌的调节活化蛋白表达增加
Rosiglitazone inhibit the upregulation of regulated upon activation, normal T cell expressed and secreted induced by advanced glycosylation end products in human renal mesangial cells
目的 探讨糖基化终产物(AGEs)对体外培养的人肾系膜细胞(HRMC)趋化因子—正常T细胞表达分泌的调节活化蛋白(RANTES)基因和蛋白表达的影响以及罗格列酮的干预效应。方法 运用糖基化修饰的牛血清白蛋白（AGE-BSA）加或不加罗格列酮干预体外培养的HRMC，用半定量RT-PCR检测细胞中RANTES mRNA水平，用Western blot和ELISA法分别测定细胞裂解物和培养上清中RANTES蛋白水平。结果 （1）AGE-BSA呈浓度和时间依赖性增加人肾系膜细胞RANTES mRNA和蛋白表达；（2）罗格列酮呈浓度依赖性降低AGE-BSA刺激的人肾系膜细胞RANTES mRNA和蛋白的表达 。结论 罗格列酮能抑制糖基化终产物诱导的人肾系膜细胞RANTES的表达和分泌。
Objectives: To investigate the effects of advanced glycosylation end products (AGEs) on the expression of chemokine RANTES (regulated upon activation, normal T cell expressed and secreted) in the cultured human renal mesangial cells (HRMC) and the effect of rosiglitazone. Methods: AGEs were prepared by incubation of bovine serum albumin(BSA) with high concentration of glucose at 37
马丽、孙子林、周莉
基础医学药学
糖基化终产物正常T细胞表达分泌的调节活化蛋白人肾系膜细胞
GEs RANTES human renal mesangial cells
马丽,孙子林,周莉.罗格列酮抑制糖基化终产物诱导的人肾小球系膜细胞正常T细胞表达分泌的调节活化蛋白表达增加[EB/OL].(2008-02-13)[2025-08-18].http://www.paper.edu.cn/releasepaper/content/200802-111.点此复制
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