M3受体与β-catenin在大鼠心室肌结构共定位关系的研究

im To study structural integration between M3 muscarinic acetylcholine receptors (M3-mAChR) and β-catenin in parts of cell membranes in isolated myocytes and explore the new target drug action for antiarrhythmia.Methods Immunostaining the single normal ventricular myocytes were introduced to determin the distribution of M3-mAChR and β-catenin. Immunoprecipitation was used to demonstrate that the colocalization between M3-mAChR and β-catenin in response to the ischemia time developing. Results β-catenin was closely associated with M3-mAChR in parts of cell membranes in isolated Myocytes. At the same time, the colocalization was closer in response to the ischemia time developing and choline partially strengthened the ischemia-induced activation of the association between β-catenin and M3-mAChR. Conclusion The structural integration between M3-mAChR and β-catenin in rat ventricular myocardium,and this integration could be strengthened by administration with choline.