新加良附方抑制人胃癌细胞增殖的分子机制研究

Objectives： To explore the inhibitory effect of XinJiaLiangFuFang serum and Dioscin serum on the proliferation of human gastric cancer cells BGC-823 and the possible mechanism of their action. Methods: To prepare XinJiaLiangFuFang serum and Dioscin serum and act on the human gastric cancer cells BGC-823 and detect the cell proliferation inhibitory rate using MTS assay.To observe the effect of XinJiaLiangFuFang serum and Dioscin serum on the expression of caspase-3 and p53 in human gastric cancer cells BGC-823 using RT-PCR method.To detect the expression level of miR-34a in gastric cancer cell using RT-PCR method. Results XinJiaLiangFuFang serum and Dioscin serum have significant cell proliferation inhibitory effect against the human gastric cancer cells BGC-823 and its effect is directly proportional to time. The proliferation inhibitory effect of XinJiaLiangFuFang serum is better than Dioscin serum at 48h and 72h.The expression level of caspase-3 in human gastric cancer cells BGC-823 is lower than the normal gastric mucosa epithelial cells GES-1,while its p53 expression level is higher than the normal GES-1 cell. After the XinJiaLiangFuFang serum and Dioscin serum have acted on the BGC-823 cell, its caspase-3 gene expression level significantly increased and the expression level has increased with time,while the p53 gene expression level decreased and is inversely proportional to time.The regulation of caspase-3 and p53 gene between XinJiaLiangFuFang serum and Dioscin serum has no statistical difference.The expression level of miR-34a in gastric cancer cell is higher than the normal gastric mucosa epithelial cells GES-1. Conclusions: XinJiaLiangFuFang and Dioscin can significantly inhibit the proliferation of human gastric cancer cells BGC-823 and their effect may be related to the increase of caspase-3 expression and the inhibition of mutated p53 expression.MiR-34a might play a role of oncogene and it is necessary to study further for the next research.