榄香烯增强胰腺癌细胞吉西他滨敏感性的实验研究

Objective: To explore the efficacy of elemene in combination with gemcitabine on pancreatic cancer Bxpc-3 cells. Methods: The growth inhibitory effects of elemene and gemcitabine alone or combination on Bxpc-3 cell line were assayed with MTT test. The equivalent method line method was employed to observe whether combination shown synergistic effect. Apoptosis rates were quantitatively determined by AnnexinV/PI double staining. Results: elemene and gemcitabine combination treatment on Bxpc-3 cell line for 48hr（IC50=0.063±0.01μg/mL）could reduce the IC50 of gemcitabine treatment alone for 48hr（IC50=0.31±0.04 μg/mL）significantly. Meanwhile the combination treatment shown synergistic effect. The occurrence of apoptosis was detected with a dosage-dependent and time-dependent manner by AnnexinV/PI double staining,. Conclusion: elemene in combination with Gemcitabine can effectively inhibit the growth and induce cells apoptosis in Bxpc-3 cells.