利用cDNA array分析水稻中具昼夜节奏及生物钟变化的基因
Identification of diurnal and circadian candidate genes in rice by using cDNA array
用5837条具3’端Poly(A+)的非冗余的表达序列标签（EST）的水稻cDNA 阵列，用它检测一昼夜内不同光照条件下间隔4小时的基因表达谱。实验分离出190条具有昼夜节奏表达特性的基因，进一步分析发现其中有170条基因的表达可能受生物钟调控。这些具有昼夜节奏和生物钟表达特性的基因参与了广泛的生理过程，包括光合作用、糖酵解、水和矿质元素运输、基因的转录和翻译等等，这表明生物钟在协调植物体内的各种生理中起着重要的作用。通过对全部具昼夜节奏基因的聚类分析，我们发现这些基因存在10种不同的昼夜表达模式，这为理解昼夜节奏和生物钟调控提供重要的线索。
cDNA array containing 5837 rice unique genes was used to explore gene expression patterns at 4 h intervals throughout a day. It was found that there were 190 genes with diurnal changes, of which 170 genes could change in circadian manner. Analysis of those genes showed that circadian clock played a key role in coordination of many physiological processes such as glycolysis, water and mineral elements transport. By clustering diurnal change genes from cDNA data, we found 10 clusters with different expression patterns.
张峰、董海涛、Dean Jiang、Debao Li
分子生物学植物学生理学
水稻cDNA 阵列日变化昼夜节奏
Oryza sativa L. cDNA array Diurnal change Circadian rhythm
张峰,董海涛,Dean Jiang,Debao Li.利用cDNA array分析水稻中具昼夜节奏及生物钟变化的基因[EB/OL].(2005-12-30)[2025-08-03].http://www.paper.edu.cn/releasepaper/content/200512-788.点此复制
评论