Biofilm matrix proteome of clinical strain of P. aeruginosa
Biofilm matrix proteome of clinical strain of P. aeruginosa
Abstract Extracellular matrix plays a pivotal role in biofilm biology. Despite importance of matrix proteins as potential targets for development of antibacterial therapeutics little is known about matrix proteomes. While P. aeruginosa is one of the most important pathogens with emerging antibiotic resistance only few studies are devoted to matrix proteomes and there are no studies describing matrix proteome for any clinical isolates. As matrix responsible for some extracellular functions, it is expected that protein composition should be different in comparison with embedded in biofilm cells and this difference reflects possible active processes in matrix. Here we report the first matrix proteome for clinical isolate of P. aeruginosa in comparison with embedded cells. We have identified the largest number of proteins in matrix among all published studies. Ten proteins were unique for matrix and not present inside cells, but most of these proteins do not have well described function with respect to extracellular component of biofilm. Functional classification of enriched in matrix proteins resulted in several bioprocess groups of proteins. Top three groups were: oxidation-reduction processes, nucleoside metabolism and fatty acid synthesis. Finally, we discuss obtained data in prism of possible directions for antibiofilm therapeutics development.
Egorova Daria A.、Danilova Ksenya A.、Dmitrieva Maria A.、Tutykhina Irina L.、Scherbakova Anastasya A.、Ginsburg Alexander L.、Solovyev Andrey I.、Kravtsov Ivan N.、Polyakov Nikita B.、Romanova Yulia M.
National Research Center of Epidemiology and Microbiology n. a. N.F. Gamaleya, Russian Ministry of HealthNational Research Center of Epidemiology and Microbiology n. a. N.F. Gamaleya, Russian Ministry of HealthNational Research Center of Epidemiology and Microbiology n. a. N.F. Gamaleya, Russian Ministry of HealthNational Research Center of Epidemiology and Microbiology n. a. N.F. Gamaleya, Russian Ministry of HealthNational Research Center of Epidemiology and Microbiology n. a. N.F. Gamaleya, Russian Ministry of HealthNational Research Center of Epidemiology and Microbiology n. a. N.F. Gamaleya, Russian Ministry of Health||I.M. Sechenov First Moscow State Medical UniversityNational Research Center of Epidemiology and Microbiology n. a. N.F. Gamaleya, Russian Ministry of HealthNational Research Center of Epidemiology and Microbiology n. a. N.F. Gamaleya, Russian Ministry of HealthNational Research Center of Epidemiology and Microbiology n. a. N.F. Gamaleya, Russian Ministry of HealthNational Research Center of Epidemiology and Microbiology n. a. N.F. Gamaleya, Russian Ministry of Health||I.M. Sechenov First Moscow State Medical University
基础医学微生物学分子生物学
P. aeruginosamatrixomeproteomematrixbiofilmclinical isolatecystic fibrosis
Egorova Daria A.,Danilova Ksenya A.,Dmitrieva Maria A.,Tutykhina Irina L.,Scherbakova Anastasya A.,Ginsburg Alexander L.,Solovyev Andrey I.,Kravtsov Ivan N.,Polyakov Nikita B.,Romanova Yulia M..Biofilm matrix proteome of clinical strain of P. aeruginosa[EB/OL].(2025-03-28)[2025-05-06].https://www.biorxiv.org/content/10.1101/2021.12.21.473640.点此复制
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