黄曲霉素调控β1,4-半乳糖基转移酶 I 表达
Identification of β1,4-galactosyltransferase I As a target gene of Aflatoxin B1
目的: 初步研究肝癌相关因子黄曲霉素对糖链合成酶β1,4-半乳糖基转移酶 I 的表达调控。方法：利用免疫组化检测正常组织和肝癌组织β1,4-半乳糖基转移酶 I 的表达的差异；利用RT-PCR和双荧光素酶报道基因活性测定黄曲霉素处理前后β1,4-半乳糖基转移酶 I mRNA的表达和启动子活性。结果：临床标本表明，肝癌组织β1,4GT I 的表达明显高于正常对照，而且其细胞膜蛋白的Galβ1→4GlcNAc 修饰增强。肝癌病人血清的β1,4GT I 活力在术后明显下调，但肝癌复发后，β1,4GT I 活力又明显上调。肝癌发生相关因素黄曲霉素可上调GalT I 的表达，E2F1 介导了黄曲霉素对β1,4GT I 的转录调控机制。结论: β1,4-半乳糖基转移酶 I在肝癌中高表达；黄曲霉素通过E2F1转录因子调控β1,4-半乳糖基转移酶 I 表达。
Objective To investigae the effec of Aflatoxin B1 (AFB1) exposure, an etiological factors associated with hepatocellular carcinoma on the expression of β1,4-galactosyltransferase I. Methods Immunohistochemistry method was used to detect the expression of GalT I in hepatoma tissues and normal tissues. And, double luciferase reporter analysis was used to detect the effect of Aflatoxin B1 on GalT promoter activity. Results β1,4-galactosyltransferase I (GalT I) is significantly up-regulated in hepatoma, and is transcriptionally regulated by AFB1. The AFB1-induced GalT I transcription is mediacted by E2F1 transcription factor. Conclusions These results provide genetic evidence that GalT I upregulation mediated by E2F1 might contribute to hepatoma development mediated by AFB1.
江建海、顾建新、魏湲颜
基础医学肿瘤学分子生物学
肿瘤糖生物学β14-半乳糖基转移酶 I黄曲霉素肝癌
he glycobioogy of tumorHepatomaflatoxin B1β14-galactosyltransferase I
江建海,顾建新,魏湲颜.黄曲霉素调控β1,4-半乳糖基转移酶 I 表达[EB/OL].(2011-01-10)[2025-08-21].http://www.paper.edu.cn/releasepaper/content/201101-427.点此复制
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