妊娠期糖尿病可增加子代大鼠焦虑行为
Prenatal exposure to gestational diabetes mellitus can increase the anxiety behavior of offspring rats
【目的】研究妊娠期糖尿病（GDM）对子代行为的影响，初步探讨其机制。【方法】孕鼠尾静脉注射链脲佐菌素建立大鼠GDM模型。分别于子代2月、4月及10月龄时进行黑白箱、旷场、高架十字迷宫 （EPM）及糖水偏好等行为学检测。收集子代大鼠脑前额叶皮层，检测5-HT1A蛋白及mRNA表达水平。【结果】除旷场移动总距离外，GDM 4月及10月龄子代大鼠各行为学实验各项指标与正常同龄子代均有显著差异。GDM 2月龄子代除进入EPM开放臂次数明显减少外，其他行为学指标与正常同龄子代无显著差异。GDM各年龄段子代脑前额叶皮层5-HT1A的mRNA水平及4月和10月龄子代5-HT1A蛋白表达水平均较正常同龄子代明显降低。2月龄子代5-HT1A蛋白表达两组间无显著差异。【结论】GDM可增加子代焦虑行为，其机制与GDM致子代脑5-HT1A蛋白及mRNA水平下降有关。
In this paper, the behavior change and the mechanisms were determined in the offspring prenatal exposure to gestational diabetes mellitus. Pregnant rats injected streptozotocin by tail vein. Behavior test were carried on the offspring rats when they were 2 months, 4months and 10 months old, including Black and White Box, Open Field, Elevated Plus-Maze and saccharine preference. 5-HT1A mRNA and protein level of the prefrontal cortex were assessed. Except the move distance in the Open Field, 4months and 10 months old GDM offspring were all changed in all behavior test significantly. 2 months old GDM offspring significantly decreased the times of open-arm entries, but other behavior differences were not found. GDM offspring prefrontal cortex 5-HT1A mRNA were all down regulated. 5-HT1A protein were down regulated too, except 2 months old GDM offspring. Prenatal exposure to gestational diabetes mellitus could increase anxious behavior of offspring, probably via down-regulation 5-HT1A mRNA and protein.
曹莉、姜华凤、镇学初
基础医学妇产科学神经病学、精神病学
神经药理学妊娠期糖尿病子代大鼠焦虑5-HT1A受体
neuropharmacologygestational diabetes mellitusoffspringratanxiety5-HT1A receptor
曹莉,姜华凤,镇学初.妊娠期糖尿病可增加子代大鼠焦虑行为[EB/OL].(2016-09-09)[2025-08-02].http://www.paper.edu.cn/releasepaper/content/201609-77.点此复制
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