药用植物穿心莲离体培养技术及其应用研究进展

ndrographis paniculata is one of the most important Southern Medicines in China.It is used for clearing heat and detoxifying, cooling blood and reducing swelling. Its main activeingredient, andrographolide, functions in anti-cancer, anti-HIV, anti-inflammation and liverprotection. It is difficult to be synthesized artificially, mainly relying on extraction from cultivatedplant materials. However, the quality of cultivated medicinal materials is affected by variousfactors such as soil, climate, water and fertilizer management. On the other hand, A. paniculatahas a long growth cycle, occupying land resources. The technology of plant in vitro culture hassignificant advantages in rapid propagation of seedling and accumulation of active ingredients,which is one of the important ways to achieve production of active ingredients rapidly and efficiently in A. paniculata. The in vitro regeneration technology system of A. paniculata isbecoming more and more perfect, and the in vitro regeneration technology from explants tocomplete plants is becoming more and more mature, it has been applied in seedling propagationand ploidy breeding. At the same time, during callus culture, cell suspension culture, adventiticroot culture and hairy root culture of A. paniculata, the accumulation of andrographolide and otheractive ingredients in the culture things could be greatly increased by optimizing the cultureconditions and using appropriate inducers. This paper comprehensively and systematicallyreviewed the research advances in the technology of in vitro culture of A. paniculata andproduction of andrographolide from the aspects of tissue culture, cell culture, adventitious rootculture, and hairy root culture. This paper aimed to provide reference for promoting thedevelopment and application of in vitro culture technology of A. paniculata, as well as for thestudy of in vitro production of andrographolide. It also put forward three aspects that need befocused on in future research on in vitro culture technology of A. paniculata and the production ofandrographolides by this technology: (1) to mature and improve the tissues in vitro regenerationtechnology system of A. paniculata, and establish a comprehensive and systematic evaluationsystem; (2) to further increase the yield of andrographolide and other important active ingredientsby optimizing the culture conditions and its combination with efficient inducers; and (3) to carryout researches in bioreactor culture of the production of andrographolide by cell suspensionculture technology.