复方银黄微型灌肠剂对大鼠腹腔巨噬细胞NO及iNOS的影响

IM To study the effect of Yinhuang Micro-enema Compound(YHMCE) on release of nitric oxide(NO) and the activity of induced nitricoxide synthase(iNOS) so as to elucidate its anti-inflammatory mechanisms. METHODS Peritoneal macrophage(PMФ) obtained from normal rats were cultivated in vitro. The production of NO，the activity and mRNA expression of iNOS in peritoneal macrophages of mice administered with different dose of YHMCE were observed by nitrate reductase method，immunocytochemical method and RT-PCR，respectively. RESULT　YHMCE can inhibit the activity and mRNA expression of iNOS with dose-dependence in rat peritoneal macrophages, and reduced the generation and release of NO. CONCLUSION The regulation of YHMCE on the production of NO in peritoneal macrophages of mice may occur at transcriptional level of iNOS, inhibiting the iNOS’s denovo synthesis and reducing the the generation and release of NO .This may be one of its anti-inflammatory mechanisms.