P敏感的钾通道开放剂对离体肺保护的试验研究

Objective:To investigate the protective effect of pinacidil, an ATP regulated potassium channel opener on isolated lung. Methods: 15 hybrid gray rabbits were devided into 3 groups randomly. An isolated recirculating blood perfused ventilated rabbit model were used.Control group(n=6) underwent immediate reperfused for 2 hours.Ischemia group(n=6) were flushed with LPD solution + DMSO,stored at 4℃ for 8 hours and then reperfused for 2 hours.Pinacidil protected group (n=6)were flushed with LPD+ pinacidil 100μmol/L ( soluted in DMSO) , stored at 4℃ for 8 hours and then reperfused for 2 hours. Every 30 minutes, intermittent blood gases were taken from the pulmonary artery and left atrium to evaluate the function of lungs. The histologic injury of lung samples ,the wet-to-dry ratios and the content of SOD, TNF-α,IL-8 in the homogenate of lung were measured. Results: The ΔPo2 and SOD in pinacidil group was significantly higher than in control group(P＜0.05). The histologic injury in pinacidil group was less than in control group. The concentration of TNF-αand IL-8 in pinacidil group was significantly lower than in control group(P＜0.05). Conclusions: The protective effects of Pinacidil in conjunctions with LPD solution on isolated lung were better during the 8 hours preservation.