肥胖人群与正常人群尿液蛋白质组的比较

Objective: Comparison of urine proteome between obese people and normal people.Methods: Urine samples from obese and normal people were collected and identified by non-label quantitative proteomics using high performance liquid chromatography tandem mass spectrometry (LC-MS/MS). The difference proteins of urine proteome between obese and normal people were screened for protein function and biological pathway analysis. The urine proteome of obese individuals was compared with that of normal people, and the common differential proteins were counted to analyze the protein function and biological pathways. Reported biomarkers of obesity were searched in the urine proteome of obese individuals.Results: 38 different proteins can be identified in the urine proteome of obese people compared with normal people, some of which have been reported to be related to metabolism and obesity, and the biological processes of differential proteins are also related to metabolism and other processes. 8 common differential proteins in the urine proteome of obese individuals and normal people, among which some proteins have been reported to be related to metabolism and obesity, and the biological processes of differential proteins are also related to metabolism and other processes. Among the differential proteins in the urine proteome of obese individuals compared with the normal people, the reported obesity biomarkers can be matched.Conclusions: The urine proteome can distinguish the obese people, and the differential proteins in the urine proteome have key proteins that are known to be related to obesity and metabolism, and the biological processes of differential proteins also related biological processes such as nutrition and metabolism. Urine proteome has the potential to explore the pathogenesis of obesity and provide personalized treatment.