谷氨酸棒杆菌DS酶的基因克隆及L183Q定点突变
loning and Site-specific Mutagenesis of DS Enzyme gene of Corynebacterium glutamicum
目的：通过点基因工程技术获得催化活力提高的谷氨酸棒杆菌DS酶。方法：采用定点突变的方法获得正向突变的，重组酶活力提高的谷氨酸棒杆菌DS酶。结果：对谷氨酸棒杆菌LG-332中DS酶基因进行了定点突变，获得了L183Q突变体,为高活力DS酶基因工程菌的获得奠定了基础。
Object:To obain the higher activity DS enzyme through genetic engineering technology.Method:Point mutation is used to gain a higher activity recombinat DS enzyme from Corynebacterium glutamicum.Result:The point 183 was mutated from L to Q in LG-322 Corynebacterium glutamicum, it is a fundermental of construct a genetic engineering which has high activity DS enzyme.
刘俊梅、胡耀辉、王玉华、于寒松
生物工程学分子生物学遗传学
发酵工程谷氨酸棒杆菌氨基酸合成关键酶
Fermentation EngineeringCorynebacterium glutamicumkey enzyme of amino acid synthesize
刘俊梅,胡耀辉,王玉华,于寒松.谷氨酸棒杆菌DS酶的基因克隆及L183Q定点突变[EB/OL].(2012-05-17)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/201205-279.点此复制
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