Slit2对成纤维肉瘤血管新生的影响

xon guidance molecules play an important role in axonal growth, migration and orientation. In recent years,axon guidance molecules are also involved in the regulation of vascular growth, but with much controversy. In this study, using RT-PCR, the expression of several axon guidance molecules that had been confirmed previously playing regulatory roles in vascular development are examined in tumor cells. We show that fibrosarcoma HT1080 cells maintain a relative higher expression of slit2, whereas no expression is detected in lung cancer cells, A549. In order to further examine the effect of Slit2 on HT1080 induced angiogenesis, co-culture of normal fetal lung fibroblasts (HFL-1) and human umbilical vein endothelial cells (HUVEC) is used to induce angiogenesis, in which HT1080 cell conditioned medium acts as pro-angiogenic activator. The blockage of slit2 by antibody promotes HT1080 cell conditioned medium induced angiogenesis, thus suggesting a negative control of Slit2 in fibrosarcoma induced angiogenesis.