猪Myf6基因3'UTR序列的克隆及多态性分析
loning and polymorphism analysis of 3'UTR of porcine Myf6 gene
目的：克隆猪Myf6基因的3'UTR，获得其完整的cDNA序列，寻找myf6基因中存在的SNPs。方法：采用电脑克隆和RACE技术，克隆Myf6 基因的3'UTR 序列和DNA序列。以华中农业大学试验猪场2002年屠宰的大白猪、长白猪和梅山猪等三个品种和大白猪×长白猪、长白猪×大白猪、大白猪×梅山猪、梅山猪×大白猪等四个杂交组合群体（共321 头）以及2000、2003、2004 年屠宰的大白猪×梅山猪杂种猪F2 代群体（共393头）为试验猪群，对猪Myf6基因3'UTR中存在的SNP进行了验证与基因型分型。结果：扩增出了Myf6 基因3'UTR 长638bp， GenBank 登录号为DQ139774。在该区域发现一处A/C突变引起了酶切位点TasⅠ的改变，在8个群体中检测了该位点的基因型频率分布。结论：获得猪Myf6基因的3'UTR，对其中存在的SNP进行了基因分型和基因型频率检测。
IMS: To clone 3'UTR sequence and find SNPs in the the porcine Myf6 gene. METHODS: Using rapid amplification of cDNA ends (RACE) technology, we obtained 3'UTR of Myf6 gene. SNPs in porcine Myf6 gene were studied in different pig breeds by sequencing, RESULTS: we obtain the 3'UTR of the porcine Myf6 gene, GenBank accession number DQ139774, 3'UTR 638bp. 8 SNPs were obtained in the porcine Myf6 gene. Of 4 are transversion mutations, 4 are transition mutations. One novel SNP, an A/C substitution in the 3'UTR of Myf6 gene, was found by sequencing. The SNP could be detected using the TasI restriction enzyme. We analyzed the distributions of genotypes in different pig populations. CONCLUSION: The 3'UTR of porcine Myf6 gene was successfully cloned. Allele frequencies and distributions of genotypes for this SNP were studied in different pig populations.
彭健、刘敏、蒋思文、徐德全
遗传学分子生物学
动物遗传育种猪Myf6基因3'UTR基因分型
animal genetics and breedingporcineMyf6 gene3'UTRGenotype
彭健,刘敏,蒋思文,徐德全.猪Myf6基因3'UTR序列的克隆及多态性分析[EB/OL].(2013-01-04)[2025-08-16].http://www.paper.edu.cn/releasepaper/content/201301-98.点此复制
评论