BAMBI在C2C12成肌分化中的作用

BAMBI (BMP and Activin receptor Membrane Bound Inhibitor) is widely involved in numerous biological processes, either through inhibiting TGFβ activity as a decoy receptor or enhancing canonical Wnt/β-catenin pathway. While its role during myogenesis remains largely unknown. In our study, BAMBI was sharply increased on the onset of myogenic differentiation, and then gradually declined. BAMBI siRNA powerfully reduced the expression level of myogenic markers, such as MyoD、MyoG and MyHC, indicated by real-time PCR and western-blot. Accordingly, cell immunochemistry assay shown a decreased number of fused myotubes, differentiation index and fusion index, which was a phenomenon of a repressed myogenic differentiation. Our data suggested BAMBI might promote C2C12 differentiation, and the underlying mechanism needs more inspection.