Diverse laboratory colonies of Aedes aegypti harbor the same adult midgut bacterial microbiome
Diverse laboratory colonies of Aedes aegypti harbor the same adult midgut bacterial microbiome
Abstract BackgroundHost-associated microbes, collectively known as the microbiota, play an important role in the biology of multicellular organisms. In mosquito vectors of human pathogens, the gut bacterial microbiota influences vectorial capacity and has become the subject of intense study. In laboratory studies of vector biology, genetic effects are often inferred from differences between geographically and genetically diverse colonies of mosquitoes that are reared in the same insectary. It is unclear, however, to what extent genetic effects can be confounded by uncontrolled differences in the microbiota composition among mosquito colonies. To address this question, we used 16S metagenomics to compare the midgut bacterial microbiome of six recent laboratory colonies of Aedes aegypti representing the geographical range and genetic diversity of the species. ResultsWe found that the diversity, abundance, and community structure of the midgut bacterial microbiome was remarkably similar among the six different colonies of Ae. aegypti, regardless of their geographic origin. We also confirmed the relatively low complexity of bacterial communities inhabiting the mosquito midgut. ConclusionsOur finding that geographically diverse colonies of Ae. aegypti reared in the same insectary harbor a similar gut bacterial microbiome supports the conclusion that the gut microbiota of adult mosquitoes is environmentally determined regardless of the host genotype. Thus, uncontrolled differences in microbiota composition are unlikely to represent a significant confounding factor in genetic studies of vector biology.
Lambrechts Louis、Dickson Laura B.、Volant Stevenn、Mayanja Martin N.、Jiolle Davy、Paupy Christophe、Lutwama Julius J.、Kohl Alain、Ma Laurence、Duong Veasna、Dusfour Isabelle、Bouchier Christiane、Vega-R¨2a Anubis、Ghozlane Amine
Insect-Virus Interactions Group, Department of Genomes and Genetics, Institute PasteurInsect-Virus Interactions Group, Department of Genomes and Genetics, Institute PasteurBioinformatics and Biostatistics Hub, C3BI, USR 3756 CNRS, Institut PasteurDepartment of Arbovirology, Uganda Virus Research InstituteMIVEGEC, IRD, CNRS, Univ. Montpellier||Centre International de Recherches M¨|dicales de FrancevilleMIVEGEC, IRD, CNRS, Univ. Montpellier||Centre International de Recherches M¨|dicales de FrancevilleDepartment of Arbovirology, Uganda Virus Research InstituteMRC-University of Glasgow Centre for Virus ResearchGenomics Facility ¨C Biomics Pole, CITECH, Institut PasteurVirology Unit, Institut Pasteur in CambodiaVector Control and Adaptation, Institut Pasteur de la GuyaneGenomics Facility ¨C Biomics Pole, CITECH, Institut PasteurLaboratory of Medical Entomology, Environment and Health Unit, Institute Pasteur de la GuadeloupeBioinformatics and Biostatistics Hub, C3BI, USR 3756 CNRS, Institut Pasteur||Genomics Facility ¨C Biomics Pole, CITECH, Institut Pasteur
微生物学昆虫学分子生物学
MosquitoMicrobiotaVectorial capacityMetagenomics
Lambrechts Louis,Dickson Laura B.,Volant Stevenn,Mayanja Martin N.,Jiolle Davy,Paupy Christophe,Lutwama Julius J.,Kohl Alain,Ma Laurence,Duong Veasna,Dusfour Isabelle,Bouchier Christiane,Vega-R¨2a Anubis,Ghozlane Amine.Diverse laboratory colonies of Aedes aegypti harbor the same adult midgut bacterial microbiome[EB/OL].(2025-03-28)[2025-05-06].https://www.biorxiv.org/content/10.1101/200659.点此复制
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